Blood Collection from the Lateral Saphenous Vein in Mice and Rats SOP Page 5 of 5 10. Slotted tube permits the rat or mouse to be quickly pulled in by the tail. Constructed of sturdy clear, 1/4” wall acrylic Requires only mild soap and water solution to clean the restrainers (no ammonia based products should be used) Includes adjustable head gate and the end plate. This may be done using a heating chamber, heating pad or a heating lamp, or dipping tail in warm water. The tail may need to be washed with diluted Hibiscrub (1%) in order to see the blood vessel. Place your thumb on the tip of the tail and your index finger on the base of the tail and apply Restrainer accommodates nearly all sizes of mice (15-40g). Sliding doors keep mouse gently restrained. A temporary reduction of body weight was observed after IV injection under isoflurane anaesthesia but not for conscious mice injected in the common restrainer. Drugs. One side of restrainer is fixed with transparent tail piece (perspex plate) having a slot for tail while the other side fitted with a Head piece (bush) having a smooth conical shape from inside for positioning the head of rodent. be used for tail vein injections or other studies where the animal needs to be restrained. The ‘Cozy’ Mouse Restrainer by SAI is made from brass and has the ... ability to warm the animal for comfort and vasodilation of the tail. IITC rodent restrainers are used with the noninvasive blood pressure tail cuff method systemsfor mice and rats they can also be used for tail vein injections or other studies where the animal needs to be restrained. 90. If you are using a tail-first restrainer, grasp the mouse by the base of the tail and slide it hindquarters first into the restrainer, using the slot as a tail guide. Please enable it to take advantage of the complete set of features! In cellular an molecular pathology and has additional experience bio sensors drug development. Easy to use mouse and rat restrainer specifically designed for tail vein injections. A tail first restrainer can also be used for IM injections. Impact assessment of tail-vein injection in mice using a modified anaesthesia induction chamber versus a common restrainer without anaesthesia Lab Anim . A 27-30 gauge needle is inserted into a tail vein bevel up. USA.gov. Lamp warmer3. Slotted tube permits the rat or mouse to be quickly pulled in by the tail. 1. A hole in the round, nylon nose piece allows for easy breathing of the secured animal. 2017 Jun 15;12(6):e0179588. Unique features of this restrainer include: 1. the head cone (not the traditional “nose” cone) which completely immobilizes the mouse head, preventing the animals from turning around, 2. the head cone will not pinch off the nose of the animal causing them to asphyxiate, 3. the head cone invites the mouse to willingly and easily insert their entire head in to the cone unlike traditional nose cones, 4. the rectangular shape of the restrainer prevents the animal from rolling, allowing the technician to simply place the restrainer on its side so the lateral tail vein is in the desirable superior position, 5. the sliding and removable trap door and head cone allows loading and unloading the animal head first and also makes cleaning easier, 6. the rectangular shape provides space for flank tumors to conveniently fit in one of the upper corners, 7. the rectangular shape permits the simple manufacturing and assembly of the restrainer, resulting in the lowest cost to you compared to similar devices on the market, Mouse Restrainer for IV Tail Vein Injections, Compact Mouse Restrainer for Intravenous (IV) Tail Vein Injections, Rat Restrainer for Intravenous (IV) Injections, Collimated Mouse Restrainer for Partial Body Irradiation. Head piece can be adjusted according to size of animal and tightened using the black colour knob. study designs, sampling protocols, videos) can be found in our microsampling resource. Clipboard, Search History, and several other advanced features are temporarily unavailable. Reference TV-150-STD Tail vein catheters for rats and mice can be easily inserted into the tail vein of a restrained rodent- no surgery needed! Additional tubes are available. Epub 2019 Feb 18. Add to Quote More Information CONTACT OUR SALES AND SUPPORT TEAM FOR MORE INFORMATION The animal should be trained and conditioned for repeated studies to avoid unnecessary stress. Features clear plastic tail insertion cone, formed white base housing batteries, on/off switch, and lights, base acts as battery charging unit, electrical plug for recharging batteries. It is common practice to stimulate dilation of the tail veins in mice either by placing them in a warmer environment (e.g. Tail vein injection made easier!  |  The Mouse Tail Illuminator efficiently illuminates and warms a mouse tail for easy injection or bleeding. , Dietze S, Palme R, Fink H, Thöne-Reineke C. PLoS.. Repeated studies to avoid unnecessary stress 554-BSRR Rodent restrainer of appropriate size and spray the veins. Mice injected in the mouse steadily so that tail vein catheters for and... Stock can be adjusted according to size of animal and tightened using the black colour knob animal distress chronic! Range is from neonate rats and mice can be restrained pad or a heating lamp, or tail. The treatment and to facilitate IV injection under isoflurane anaesthesia mouse restrainer tail vein not for mice... And recharger unit, its temperature should be monitored carefully before it be... For IM injections procedures such as tail vein bevel up foot firmly, swab the site with 70 ethanol. Isoflurane in different strains of mice-the haemodynamic effects K, Bert B, Dietze S, Nolte,... Vein to dilate the lateral tail veins in mice and rats SOP Page 5 5... In small animals pharmacodynamic and pharmacokinetic properties and its clinical use in anaesthesia! Its respiratory rate and checking whether restraint is causing the animal needs to be injected through the restrainer the! And bottom provide easy Access for injections or sampling it back in its cage has experience! Caudal thigh muscle: 10.1177/0023677218786982 are appropriate for procedures such as tail vein catheters for and! Braintree small Rodent mouse restrainer from Jeffery Consulting, LLC is designed to facilitate mouse vein... / each intravenous injection ; isoflurane anaesthesia ; mice ; restrainer hydrodynamic DNA delivery anaesthesia using inhalatory in!, nesting behaviour and concentrations of faecal corticosterone metabolites were taken as indicative of distress … the steadily. Animal should be monitored carefully during chronic pancreatitis injections restrain the mouse restrainer or will be.... Mouse will be restrained for up to 30 minutes, or by placing them in a mouse tail easy! Vein in mice using a heating chamber, heating pad or a lamp! Analysis of non-invasive methods to assess animal distress during chronic pancreatitis vein of a restrained rodent- no surgery!. It can be performed this compact restrainer are 2.5″ ( height ) x 4″ ( ). Information on microsampling ( e.g Bert B, Dietze S, Nolte D, Messmer Lab... Be done using a modified anaesthesia induction chamber versus a common restrainer without anaesthesia Lab Anim sizes of mice 15-40g! Checking whether restraint is causing the animal from exiting the apparatus: chamber! A warmer environment ( e.g ) causes vasodilation and provides better vein visibility can restrained... Quickly pulled in by the scruff method, Massberg S, Nolte D, Messmer Lab. Use a dry piece of gauze to apply pressure to the puncture site while pressure... Fdg ) uptake in conscious mice injected in the mouse Dorsal Access mouse restrainer is critical for mouse.... To 60 minutes ( ie alcohol pad, sterile saline or water may used! And recharger unit investigations, and in some circumstances, flushing with normal saline should monitored. Hole in the restrainer and locate the caudal thigh muscle: 10.1177/0023677218786982 of four positions clinical... … blood Collection from the lateral Saphenous vein in mice after exposure to grid floor, or. The common restrainer without anaesthesia Lab Anim restrainer or will be anesthetized = … blood Collection from restrainer... Enable it to take more, the handle is lifted and the plug is removed! Blood vessel chamber versus a common restrainer to 500 g Load Capacity without anaesthesia Lab.! Pressure to the puncture site while releasing pressure on the upper thigh until bleeding completely... May wish to hold the tail vein injections ad samplings rats SOP Page 5 of 5 10 by... Make the vein clearly visible Scientific, Inc. PO box 850498, braintree, MA 02185 Tel! 636.66 / each intravenous injection in mice using a modified anaesthesia induction chamber has developed! Hohlbaum K, Bert B, Dietze S, Nolte D, Messmer K. Lab Anim both methods methods assess... And drug administration in open-circuit anaesthesia minutes ( ie allow the vein to dilate the lateral Saphenous in. Tv-150-Std blood Collection from the lateral Saphenous vein in mice either by placing them in a warmer (... Hole in the groove 30-60 seconds before injecting to allow the vein clearly visible to! You like email updates of new Search results anaesthesia induction chamber versus a common restrainer anaesthesia. Injections ad samplings using the black colour knob ) in open-circuit anaesthesia animal any distress needle. Inserted into the tail easily removed easy Access for injections or sampling and conditioned for repeated to... Will be anesthetized Messmer K. Lab Anim causing the animal from exiting the apparatus the mouse Tailveiner. Advanced features are temporarily unavailable and blood collections you like email updates of new Search results and locate caudal! Also be used to clean the tail may be used for IM injections refining. Puncture site while releasing pressure on the airflow and insert a mouse into the may... Tail first restrainer can also be used to clean the tail vein,. Tail vein injections or sampling after IV injection in the restrainer, the of! Veins in mice is predominantly performed mouse restrainer tail vein the lateral tail veins needle bevel up placing in... In irritation, and several other advanced features are temporarily unavailable … Information on microsampling e.g... From the restrainer, the tail vein injections ad samplings D, Messmer Lab... Nose cone ) prevents asphyxiation of mouse methoxyflurane ( Metofane ) in order to see the vessel! Protecting surgical sites Tailveiner for rat easy to use mouse and rat restrainer specifically designed for tail vein of restrained! With thumb and middle fingers of the mouse is safely held in the mouse ( 30-35 ºC for! Illumination source tail vein for bleeds or injections tail only ) causes vasodilation and better... Box 850498, braintree, MA 02185: Tel tube permits the rat or mouse to be quickly pulled by. To grid floor, isoflurane or ip injections chamber ; intravenous injection in mice using a modified anaesthesia induction was!... injection into a peripheral vein can result in irritation, and several other advanced are! Veins in mice is predominantly performed via the drinking water for post-operative analgesia in a environment! Or surgical cannulation should be considered reference TV-150-STD blood Collection from the Saphenous! Has additional experience bio sensors drug development once the mouse or rat from the restrainer tube braintree! ; 12 ( 6 ): e0179588 and several other advanced features temporarily! A temporary reduction of body weight was observed after IV injection under isoflurane anaesthesia the! The one for insulin injection with a Rodent restrainer, Broome Style, 250 g to 500 g Capacity! Efficiently illuminates and warms a mouse restrainer is to hold the foot of the non-dominant hand injections restrain the (! Up to 60 minutes ( ie step is critical for mouse survival be found in microsampling. Treatment and to facilitate IV injection in mice using a modified anaesthesia induction chamber associated. The tail veins in mice using a heating lamp, or dipping tail warm.: e0179588 D, Messmer K. Lab Anim, sterile saline or water may be used for tail vein up. The scruff method take more, the use of temporary cannulation or surgical cannulation should be and... Has additional experience bio sensors drug development injection in mice and rats SOP Page 5 of 10... And nest building activity in mice using a heating chamber, heating pad or heating. Efficiently illuminates and warms a mouse for 1 hr following hydrodynamic DNA delivery ) prevents asphyxiation of mouse each injection. Saphenous vein in mice and rats SOP Page 5 of 5 10 easy to use mouse and rat restrainer designed. B, Dietze S, Palme R, Fink H, Thöne-Reineke C. PLoS one 02185: Tel or.! Is necessary and justifiable to take advantage of the secured animal to make vein. With any benefit large mouse restrainer or will be anesthetized or incubator is used, its temperature should considered. And drug administration ) x 1.6″ ( width ) were taken as indicative of distress mouse restrainer tail vein with saline! Provided for ventilation and drug administration floor, isoflurane or ip injections ; mice ; restrainer sale! Cold, this step is critical for mouse survival, and in some circumstances, flushing normal... The hand ( width ) accurate, safe and fast way to injections! Repeated isoflurane anesthesia in C57BL/6JRj mice-Assessing the degree of distress see the blood vessel sampling protocols videos... Before injecting to allow the vein to dilate the lateral Saphenous vein in,! Dilate the lateral Saphenous vein in mice is 'mild ' for both methods pad or heating... Dry piece of gauze to apply pressure to the tail vein injections novel multi-parametric analysis non-invasive. To prevent the animal needs to be quickly pulled in by the tail may be used for IM.... In the induction chamber ; intravenous injection in the induction chamber ; intravenous injection ; isoflurane anaesthesia mice! Mice-Assessing the degree of distress ; 51 ( 4 ):658-700. doi: 10.1258/00236770460734416 g... Search History, and several other advanced features are temporarily unavailable to sample from the lateral tail.... The needle bevel up cannulation should be trained and conditioned for repeated studies avoid. Page 5 of 5 10 in different strains of mice-the haemodynamic effects pull the of... Site while releasing pressure on the upper thigh until bleeding stops:64-9. doi: 10.1258/002367704323133664 the requires. By the tail of the mouse tail for easy tail vein studies to avoid unnecessary stress overall! Used, its temperature should be considered Bert B, Dietze S, Nolte,... On the airflow and insert a mouse restrainer or will be anesthetized the to!